Epicatechin content in green tea (Camellia sinensis) supplements and their antioxidant activities The WritePass Journal

Epicatechin content in green tea (Camellia sinensis) supplements and their antioxidant activities Introduction/background Epicatechin content in green tea (Camellia sinensis) supplements and their antioxidant activities ). Their most important role in the body is to protect cells against the effects of free radicals. Free radicals are the unstable molecular species containing an unpaired electron that are able to exist independently. Most of these species are highly reactive and are potentially damaging to the cells (Lobo, et.al. 2010). Some of the common oxygen- free radicals that are involved in many human diseases include hydroxyl radical, superoxide anion radical, hydrogen peroxide, oxygen singlet, hypochlorite, nitric oxide radical, and peroxynitrite radical. Increased levels of these reactive species in the body cells induce damage of the DNA, proteins, carbohydrates and lipids. Thus, a balance between free radicals and antioxidants is necessary for proper physiological function (Lobo, et.al. 2010). Any dis-balance in this may result the initiation of various diseases including cancer, vascular diseases and degenerative diseases. Hence, the study of antioxidants and their sources have been the focus of intense research. 1.4. Brief information on herbal supplements to use in the project This project studies the presence of epicatechin content in Camellia sinensis; thus, the project uses herbal capsules from Camellia sinensis as herbal supplements. Camellia sinenis is taken as green tea and contains antioxidants in it, which is why it is commonly used in herbal medicine. Epicatechin is abundantly found in Camellia sinensis herbal supplement. Cameillia sinensis also contains various active compounds as extracts. Some of the major active compounds found in Camellia sinensis include catechins, caffeine, flavonols and proanthocyanidins (Charles, 2013). The health benefits of green tea (Camellia sinensis) epicatechins are being extensively studied and explained . Endothelial cells are associated to vascular function and homeostasis whose dysfunction may have implication in cardiovascular disease including atherogenesis. (Moore, et.al. 2009). Moreover, some studies suggest their anticancer role by enchaining apoptosis (Ravindranath, et.al. 2009). With findings emerging fro m several new studies regarding the health benefits of the green tea from Camellia sinensis, it is being increasingly popular among the people. Methods Simple and reliable techniques will be chosen in this project to investigate epicatechin content in Camellia sinenis. Experiments will be carried out using reflux extraction to determine the biologically active compounds present in the test supplement (herbal capsules from Camellia sinensis). This will be confirmed by NMR analysis. NMR analysis will be done to confirm that determination of correct biological compound including antioxidants from Camellia sinensis. Crude extract will be made from the material extracted from herbal capsules using a suitable solvent under reflux, which will then be run on thin layer chromatography (TLC) to see if a corresponding band for the standard also appears in the extract. This will tell whether or not the compound is present in the extract, as the study of organic compounds present in the supplement of Camellia sinensis can be made using this technique. To cut of the evaporation time, rotary evaporation method will be employed while evaporating th e solvent. Reverse phase analytical high performance liquid chromatography (HPLC) will be used to identify the standard compound in the extract and then to quantify the amount of compound in it. Finally, the antioxidant assay will be carried out to determine the antioxidant activity of the extracted compound; as a control, plant Flavonoid quercetin, which has antioxidant activity, will be used. REFERENCES Charles, J.D. (2013). Antioxidant Properties of Spices, Herbs and Other Sources.  Natural Antioxidants.   39-64. Chan, E.W.C, Eu, Y.S, Tie, P.P, Law, Y.P. (2011). Pharmacognosy Research.  Antioxidant and antibacterial properties of green, black, and herbal teas of Camellia sinensis.  3(4), 266-272. Irshad, M and Chaudhuri, P.S. (2002). Indian journal of experimental biology.  Oxidant-antioxidant system: role and significance in human body..  40(11), 1233-1239. Lobo, V, Patil, A, Phatak, A, Chandra, N. (2010). Pharmacogn Rev.  Free radicals, antioxidants and functional foods: Impact on human health.  4(8), 118-126. Moore, R.J, Jackson, K.G, Minihane, A.M. (2010). The British journal of nutrition.  Green tea (Camellia sinensis) catechins and vascular function. 96(4), 597-605. Ravindranath, M.H, et.al.,. (2006). Evid Based Complement Alternat Med..  Epicatechins Purified from Green Tea (Camellia sinensis) Differentially Suppress Growth of Gender-Dependent Human Cancer Cell Lines.  3(2), 237-247. Ravindranath, et.al., (2009). Evidence-based complementary and alternative medicine.  Differential Growth Suppression of Human Melanoma Cells by Tea (Camellia sinensis) Epicatechins (ECG, EGC and EGCG).  6(4), 523-530. Roman, M, (2001)  Natural Products Insider, naturalproductsinsider.com/articles/2001/04/the-benefits-and-pitfalls-of-standardizing-botanic.aspx, 11/12/2013. Uzunalic, A.P, et.al.. (2006). Food Chemistry.  Extraction of active ingredients from green tea (Camellia sinensis): Extraction efficiency of major catechins and caffeine.  96(4), 597-605. Yang, Z, Xu, Y, Jie, G, He, P and Tu, Y. (2007). Asia Pac J Clin Nutr.  Study on the antioxidant activity of tea flowers (Camellia sinensis)  .  16(1), 148-152.

Disscuction Article Example | Topics and Well Written Essays - 250 words - 1

Disscuction - Article Example Adoption or utilization of the new concepts illustrates either positive or negative aspects of the new opinion or concept. I accept data which challenge my present views. The challenging data provides very many alternative courses of action. For example, I presently believe that the main reason for education is knowledge development for employment purposes. But there are other significant views on benefits of education such as; developing entrepreneurship potential, ensuring innovation and creativity in society, and finally realizing good political governance through developing law abiding citizens. Accepting challenging data ensures understanding of the advantages and disadvantages of the view that I illustrate. For instance, my view on affirmative action is always positive. But there are shortcomings of affirmative action, for example, giving average employees in terms of competencies senior management positions in organizations. There are ideas that can challenge the comfort level that I am in currently; for example technological innovation ideas. New technology creates new processes. The emergence of the social networks has changed the way I communicate with friends. Because of busy work and education schedule, I mostly communicate with friends via Facebook and twitter

Teenagers and pregnancy Research Paper Example | Topics and Well Written Essays - 2250 words

Teenagers and pregnancy - Research Paper Example As one of Faulkner’s most highly regarded works, the novel has the ability to incite an understanding in the reader’s mind that goes beyond the existence of our lives at face-value; it questions one’s thoughts, ideas, beliefs, values and perception even to the brink of questioning the meaning of existence. Unlike other literary pieces penned down by Faulker, As I Lay Dying is not an abstract or complex plot, which suggests the author’s creation of mind during the writing process. Cited time and time again by literature instructors and students worldwide is the one famous fact associated with the novel which also provides an ample insight into the author’s imagination – the novel took only six weeks to complete and William Faulker did not even change one single word of the manuscript upon completion. The author famously talked about how he felt completely in control of Bundrens’ lives when he penned down every agony that they were to su ffer, the novel is one-of-kind in the sense that it is one of those rare, magical and incredible instances when one experiences those rare bouts that mislead person to believe that they are masters of their own destiny, thankfully for Faulkner, this period of intense activity was translated into a prolific and fine literary specimen. Quite straightforward however, and rightfully so, As I Lay Dying is also a journey as much as it is an awakening, the journey that leads to the family’s arrival Jefferson and the burial of Addie Bundren’s body. Analyzing as to why Faulkner preferred to select breaking down his novel into fifty-nine monologues each spoken by one of the fifteen characters of the novel can be best described as an experiment in literary technique, which to put it in modest terms proved to be quite successful. Faulkner never seeks precedence of one theme or genre throughout the novel as he always has something surprising to give, which further cements the idea that the creation of the author’s mind during the process of the development of his work was clear-cut, Faulkner never seems to get lost in the premise that this story is actually the account of the deliverance of a man’s promise to his dying wife. The forty mile journey to Jefferson is a mixture of absurdity, comedy and drama with hints of a grotesque and determined effort to make Addie’s last wish come true. The family’s capricious pursuit is augmented to the nearness of a laborious ordeal with the wide character map that the author has given birth to. More than their desire to focus on the task at hand; which is the sole purpose for the trip to the town of Jefferson, each character has its own whims and yearnings (Faulkner, Random House, 1999). In the dazing context of events, Dewey Dell, the only daughter of the family has a very inconvenient issue at an inopportune time; she is seventeen, unmarried and pregnant. The purpose of this paper is to critic ally analyze and understand the psychology of the author and the creation of his mind, while understanding how Faulkner sheds light

Early Cells Essay Example for Free

Early Cells Essay 1 Hypothesis If you soak an egg in vinegar, then the shell will deteriorate and loose centimeters off of its circumference. Data and Observations Time (hrs) Observations Circumference (cm) 0 1. The egg is whole, and has not lost color. 2. The egg was immediately surrounded by bubbles. 3. 14 cm 4. 24 1. The egg appears to have a little bit of color missing. 2. The egg remains firm. 3. 15.5cm 4. 48 1. The eggs shell is beginning to disappear. 2. The shell of the egg is cracking all around. 3. 16 and  ¼cm 4. 72 1. The shell is completely gone, leaving a thin membrane that surrounds the yolk. 2. The egg itself looks flimsy and appears transparent. 3. 17cm Conclusion: Be sure to answer the following reflection questions in the conclusion of your lab report: 1. How much did the egg change in size? (answer should be in centimeter) The eggs size changed by 3 centimeters. 2. Was your hypothesis correct? Why or why not? My hypothesis proved to be correct because the eggs shell did, in fact, deteriorate. 3. Evaluate the lab and data collected. What type of transport occurred in this lab? Explain your answer, including evidence from your experiment to  support your explanation. The type of transport that took place in this lab was active. I believe this is true because the particles moved from an area of higher concentration (vinegar), to an area of lower concentration (the egg). 4. Consider how lettuce or spinach placed in water becomes firm and crisp. Use what you have learned about cell membranes to explain this observation. Lettuce and spinach become firm and crisp when placed in water because the cells absorb the water, and in turn makes the lettuce/spinach bloated with water. 5. If you were to continue this experiment by removing the egg from the water and covering it in syrup, what do you think would happen. Explain your prediction. (If you choose to test your prediction, be sure to allow at least 24 hours before making your observations. And, of course, handle the egg very carefully!) If I were to put the same egg in a syrup solution, I believe the thin membrane that remained would break because passive transport would take place.

Microsoft Office vs. OpenOffice.org Essay -- Computers Software Techno

Microsoft Office vs. OpenOffice.org According to Microsoft.com, a new version of Microsoft Office 2003 will normally cost anywhere between $400 - $500, depending on which version you get. While Microsoft also has a $150 â€Å"Student and Teacher Edition† which is the same at the $400 Standard Edition, most people do not qualify for that version, and even for those that do, $150 can still be a lot of money, especially for a student, and $400 is much more than most people would want to spend simply to type a document occasionally. These prices seem even more extreme in light of the fact that there are many free alternatives to Microsoft Office. One of the most popular and well known alternatives is OpenOffice.org, which is usually called just OpenOffice or OO. I am going to be discussing whether OO really is comparable to Microsoft Office for the average user. I'm am not going to try to show that OO is a better office suite than Office, because if you ignore the money issue, Office really is an ex cellent collection of programs that have set the standard for office suites in general. All I am going to be looking at is whether OO really has the most of the same capabilities as Office, and is comparable in it's ease of use as well. The word processor is probably the most important application to look at when comparing the two suites. Microsoft's word processor is called Word, and OO's is called Writer. These two programs are very similar in what they are able to do, and it's hard to tell much difference between the two at first glance. If a user is accustomed to Word, Writer has a very familiar look to it, and so a user new to Writer should have no problem jumping right into the program without havin... ...slide at all. That is not to say that Impress is incapable of creating custom animations, it is just that it has troubles displaying the animations from a PowerPoint file. Since the animations could be a big part of a presentation, trying to transfer PowerPoint files over to Impress files can be a lot of work. If the user does not have to transfer files, and can instead create them from scratch in Impress, there is little difference between the two programs capabilities. As we've seen, although there are some big differences between OpenOffice.org and Microsoft Office, both offer the same basic functionality to casual users who might not use some of the more complex features. Coupled with the fact that OpenOffice.org is free, and Microsoft Office can cost anywhere from $150 - $500, it seems clear that OpenOffice.org is a good choice for most people.

Women in Korean Society: A Past and Present Perspective

â€Å"The Music from Gwangsang Mountian where I played in my dream† is one of the most famous poems, which was written by a woman who lived in 1500s. The poem basically means that the writer of this poem eager to escape from the patriarchal society. The poem was written by a Korean women poet â€Å"Heo Hanselheon† who lived in 1500s wrote this poem before she died. This poem is describing when she had a dream that she was in the place called â€Å"Gwangsang Mountain†. Gwangsang Mountain is actually represents the paradise that she always dreamed of which is being free from the paternalism. In the poem, the first line, â€Å"Blue sea- water permeate into beaded sea,† show the paradise she had been dreaming, also means the scene that she saw in her dream. The second line, â€Å"Blue bird line on colored bird,† means that she is tired of all her life and she need some â€Å"rest†. The third line, â€Å"Twenty- seven lotus flower fall in red† tell us that the â€Å"rest† is death when she become a twenty-seven years old, which is predicting her death. Of coarse, red would be color of blood, which also represents her death. The last line, â€Å"It is cold on the moon light frost†, is telling that the moon light frost which represents the cruel society towards her is leading her to death or doesn’t feel any guilty and sympathy to her. In the poem, most of words are represent death of her and the dream that she had. The reason why this is important because we understand and assume how harsh her life was how much she wanted leave this horrible place. Since her poem tells us that she wants to escape from patriarchal society, so which relates to our unit 2, Hidden Voice, that there were many Korean women who had same wish like writer of this poem. On the other side, the K-pop music has totally different meanings and story than the old Korean poem. The music â€Å"I Will Show You† by Ailee is one of the music that women power gets stronger and overcome the paternalism. The story of this music is very simple; there are one man and one woman who were couple but a woman was a â€Å"nerd†. As a result, a man dumped her and gets another girlfriend. However, a woman changed into a very fashionable and confident, a man changes the attitude toward her and starts to follow her. On the other hand, a woman tired to revenge to men who made her angry and sorrow. It shows that women can control over men. In the lyric, the chorus part, â€Å"I will show you totally different myself/ I will show you more beautiful myself/ I will not cry because of you who was eager love/ I will show I am happier than you when I meet a more cool man/ I don’t feel any sadness even though you are not here, I don’t collapse, boy you gatta be aware† show how much she become confident about her appearance and herself. The starting part of the second verse, â€Å"Change my hair style more fresh and do makeup better/ everyone looking at me who wear high heels and short skirt† directly shows that how she revenge to her ex- boyfriend and it represents the higher states of women. The song is showing why this states changed in the story in the song. This song is important because in the song the states of men and women changed, how men treat women towards their appearance and why women had higher states at the middle of the song. The women and the men had different choices but ended up that she wins in this â€Å"competition† of the states. Since the poem and the song were written in different people in different time, there are many similarities and differences between two. Both poem and song is telling the story in the same point of view. These are all written in women’s perspective and it tells us the story that they have. However, there are more differences between these two. In the song, the woman tired to change her and the attitude that men had towards her. On the other hand, in the poem, the writer wanted to escape from her situation not trying to change her situation.

Parallels Between The Sun Also Rises by Hemingway and The...

Parallels Between The Sun Also Rises by Hemingway and The Great Gatsby by Fitzgerald During the decade of the 1920s, America was going through many changes, evolving from the Victorian Period to the Jazz Age. Changing with the times, the young adults of the 1920s were considered the Lost Generation. The Great War was over in 1918. Men who returned from the war had the scars of war imprinted in their minds. The eighteenth amendment was ratified in 1919 which prohibited the manufacture, sale, or transportation of liquor in the United States. Despite the eighteenth amendment, most people think of large, lavish parties when thinking about the 1920s. The nineteenth amendment was passed in 1920 which gave women†¦show more content†¦As Jones summed up on a BBS response, The certainly love drinking from dawn til dawn (Jones 1160). In comparison, many of the characters of The Great Gatsby drank excessively, keeping in mind the story was set in the United States during prohibition. Jay Gatsby, a bootlegger, threw large, lavish parties at his grand estate during the su mmer evenings where the alcohol, food and music flowed. Characters such as Tom and Daisy Buchanan, Myrtle Wilson, Jordan Baker, and Nick Carraway enjoyed parties and drank excessively. Another important parallel to point out under the vice of excessive drinking is the fact that both Hemingway and Fitzgerald drank excessively. Their excessive drinking behaviors caused their writing to suffer (McDowell 91). The second parallel between a vice in Hemingways The Sun Also Rises and Fitzgeralds The Great Gatsby is that of sexual promiscuity. The most promiscuous character in The Sun Also Rises is Brett Ashley. Nicholas McDowell described here as . . . an English aristocrat, alcoholic and promiscuous (McDowell 47). Here is a woman who is engaged to be married to Mike Campbell and had affairs with Robert Cohen and Pedro Romero while engaged. 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